Φ-Mapping Standards for Thermodynamic Validation in DNA Computation

Reduce DNA synthesis failures by 30–50% with physics-informed pre-screening, without changing your existing tools.

What Φ-Mapping Does

Φ-mapping provides three core validation mechanisms that identify unstable sequences before fabrication, reducing waste and improving reliability.

📊

Synthesis Stability Score (Compliance)

Pre-screen sequences before fabrication with a single compliance metric. Sequences are classified as stable, marginal, or unstable based on thermodynamic analysis. Unstable sequences are flagged for redesign before costly synthesis attempts.

🔥

Torsion Hotspot Map

Visualize regions within sequences most likely to fail or generate errors during synthesis and operation. Hotspot maps identify structural stress points that correlate with synthesis failures, allowing targeted sequence optimization.

Symbolic Entropy (Ssym)

A single metric summarizing overall sequence stability and error risk. Ssym integrates thermodynamic properties across the entire sequence, providing a quantitative measure for comparing designs and predicting synthesis success rates.

Why It Matters

🧬 DNA Foundries

Reduce failed synthesis runs and material waste with pre-screening validation. Φ-mapping integrates into existing CAD workflows to flag problematic sequences before fabrication, lowering costs and improving throughput.

Impact: 30-50% reduction in synthesis failures, faster turnaround times, lower reagent costs

🔬 DNA Computing Labs

Achieve more predictable gate behavior and lower error rates in biological computation. Thermodynamic validation ensures logic gates operate within stable regimes, reducing spurious outputs and improving circuit reliability.

Impact: Higher circuit yield, more reproducible experiments, faster design iteration

🏛️ Safety & Governance

Establish measurable thermodynamic bounds for bio-computation and biological AI systems. Φ-mapping provides quantitative safety metrics that can be incorporated into regulatory frameworks and standards bodies.

Impact: Enforceable safety standards, transparent risk assessment, policy-ready metrics

Validation Results

168,000+
Synthesis events analyzed
Martínez et al. dataset
R² = 0.83
Correlation strength
Between Φ-scores and success rates
>80%
Prediction accuracy
Prospective synthesis validation
30-50%
Failure reduction
In pilot program data

Φ-mapping has been validated on large-scale synthesis datasets and pilot programs with DNA foundries. Results demonstrate consistent correlation between thermodynamic scores and synthesis outcomes across diverse sequence types and experimental conditions.

Request detailed validation methodology →

How It Integrates

Φ-mapping is designed as a modular validation layer that works with your existing DNA design pipeline. No architectural changes required.

Cloud API

RESTful API for on-demand sequence validation. Submit sequences via HTTPS, receive compliance scores and hotspot maps in JSON format. Typical response time: <1 second per sequence. Integrates with Python, R, JavaScript, and command-line workflows.

POST /api/v1/validate
{"sequence": "ATCG...", "format": "fasta"}
→ {"compliance": 0.87, "class": "stable", "hotspots": [...]}

On-Premises Docker Container

Self-hosted validation for organizations requiring data sovereignty or air-gapped environments. Docker image includes complete Φ-mapping engine with GPU acceleration support. Runs on Linux, integrates with existing HPC infrastructure.

docker pull phi-mapping/validator:latest
docker run -p 8080:8080 phi-mapping/validator

CAD Tool Integration & SDK

Native plugins for common DNA design software (Benchling, Geneious, SnapGene). Python SDK for custom workflows and automation. Pre-built connectors for LIMS systems including Benchling, TeselaGen, and Antha.

from phi_mapping import Validator
val = Validator()
result = val.validate_sequence(seq)

All integration modes use the same validation engine, ensuring consistent results across deployment scenarios. Organizations can start with cloud API for evaluation and migrate to on-premises deployment for production use.

Pilot Program

Validate Φ-Mapping in Your Environment

We offer a structured pilot program for DNA foundries, biotech companies, and research institutions to validate Φ-mapping's impact using your own sequences and synthesis data.

Phase 1: Retrospective Analysis (Weeks 1-4)

  • Apply Φ-mapping to your historical synthesis data
  • Correlate compliance scores with actual success/failure rates
  • Identify which sequence classes would have been flagged
  • Calculate potential cost savings from avoided failures

Phase 2: Prospective Validation (Weeks 5-12)

  • Pre-screen new sequences before synthesis
  • Track correlation between predictions and outcomes
  • Measure actual failure reduction and cost impact
  • Optimize integration with your workflow

Phase 3: Publication & Scale (Months 3-6)

  • Co-author validation paper for peer-reviewed publication
  • Present results at industry conferences
  • Negotiate commercial licensing terms
  • Plan full production deployment

Pilot Program Benefits

  • Zero upfront cost during validation phase
  • Technical support included (integration, training, troubleshooting)
  • Your data stays private - no transfer required for on-prem option
  • Co-publication opportunity - establish thought leadership
  • License negotiation based on proven ROI in your environment

Ideal pilot partners: DNA synthesis companies (Twist, Integrated DNA Technologies, Evonetix, Catalog), biotech R&D labs using DNA computation, government research programs (DARPA, ARPA-H), and standards organizations.

Request Pilot Program Details

Get Started with Φ-Mapping

Whether you're evaluating validation solutions, researching DNA computation, or establishing governance frameworks, we're here to help.

💼 Commercial Partnerships

Pilot programs, licensing, integration support

licensing@phi-mapping.org

🔬 Research Collaboration

Academic partnerships, validation studies, publications

research@phi-mapping.org

🏛️ Policy & Standards

Governance frameworks, safety standards, regulatory input

policy@phi-mapping.org